Source: Joshua J. Timmons et al. Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs. J. Vis. Exp. (2016)
In this video, melanoma cells are intravenously injected through the tail vein in the mouse model. The melanoma cells further travel through the circulatory system, adapting to the distant microenvironment and causing metastasis in organs like lungs.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of B16-BL6 Cells
2. Tail Vein Injection
3. Counting the Lung Foci
The authors have nothing to disclose.
Trypsin | Corning | MT25052CV | |
RPMI 1640 Medium | Corning | MT15040CM | |
Phase Contraast Hemacytometer | Hausser | 02-671-54 | |
Micro-Fine IV Insulin Syringes | BD | 14-829-1D | |
Sterile Alcohol Prep Pads | Fisherbrand | 22-363-750 | |
Mouse Restrainer | Braintree Scientific | NC9999969 | Restrainer choice depends on age/size of mice |
Heating Pad | Harry Schein | NC0012697 | Optional |