Atsushi Kuno Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute National Institute of Advanced Industrial Science and Technology Biography Publications Institution JoVE Articles Atsushi Kuno has not added a biography. If you are Atsushi Kuno and would like to personalize this page please email our Author Liaison for assistance. Publications Glycosylation of Recombinant Adeno-associated Virus Serotype 6 Molecular Therapy. Methods & Clinical Development. Jun, 2024 | Pubmed ID: 38774582 Role of Wisteria Floribunda Agglutinin Binding Glycans in Carcinogenesis and Metastasis of Cholangiocarcinoma Histochemistry and Cell Biology. May, 2024 | Pubmed ID: 38393396 Differential Glycoform Analysis of MUC1 Derived from Biological Specimens Using an Antibody-Overlay Lectin Microarray Methods in Molecular Biology (Clifton, N.J.). 2024 | Pubmed ID: 38347414 Chondroitin Sulfate Modification of CSPG4 Regulates the Maintenance and Differentiation of Glioma-initiating Cells Via Integrin-associated Signaling The Journal of Biological Chemistry. Mar, 2024 | Pubmed ID: 38309500 An Improved Evanescent Fluorescence Scanner Suitable for High-resolution Glycome Mapping of Formalin-fixed Paraffin-embedded Tissue Sections Analytical and Bioanalytical Chemistry. Nov, 2023 | Pubmed ID: 37395746 Reduction in Disialyl-T Antigen Levels in Mice Deficient for Both St6galnac3 and St6galnac4 Results in Blood Filling of Lymph Nodes Scientific Reports. Jun, 2023 | Pubmed ID: 37386100 ST6GAL1 Inhibits Metastasis of Hepatocellular Carcinoma Via Modulating Sialylation of MCAM on Cell Surface Oncogene. Feb, 2023 | Pubmed ID: 36528750 Tissue Glycome Mapping: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections Methods in Molecular Biology (Clifton, N.J.). 2022 | Pubmed ID: 34972936 O-glycosylated HBsAg Peptide Can Induce Specific Antibody Neutralizing HBV Infection Biochimica Et Biophysica Acta. General Subjects. Jan, 2022 | Pubmed ID: 34582939 N-glycan Structures of Wisteria Floribunda Agglutinin-positive Mac2 Binding Protein in the Serum of Patients with Liver Fibrosis† Glycobiology. Nov, 2021 | Pubmed ID: 34192302 LM-GlycomeAtlas Ver. 2.0: An Integrated Visualization for Lectin Microarray-based Mouse Tissue Glycome Mapping Data with Lectin Histochemistry Journal of Proteome Research. Apr, 2021 | Pubmed ID: 33657805 -Glycan-Altered Extracellular Vesicles: A Specific Serum Marker Elevated in Pancreatic Cancer Cancers. Aug, 2020 | Pubmed ID: 32878320 C-Terminally Tagged NA in Replication-competent Influenza A Viruses Reveals Differences in Glycan Profiles Between NA and HA The Analyst. Aug, 2020 | Pubmed ID: 32830838 Screening SiRNAs Against Host Glycosylation Pathways to Develop Novel Antiviral Agents Against Hepatitis B Virus Hepatology Research : the Official Journal of the Japan Society of Hepatology. Oct, 2020 | Pubmed ID: 32738016 Multi-serum Glycobiomarkers Improves the Diagnosis and Prognostic Prediction of Cholangiocarcinoma Clinica Chimica Acta; International Journal of Clinical Chemistry. Nov, 2020 | Pubmed ID: 32659223 Comparative Glycomic Analysis of Exosome Subpopulations Derived from Pancreatic Cancer Cell Lines Journal of Proteome Research. Jun, 2020 | Pubmed ID: 32338917 Discovery of Pancreatic Ductal Adenocarcinoma-Related Aberrant Glycosylations: A Multilateral Approach of Lectin Microarray-Based Tissue Glycomic Profiling With Public Transcriptomic Datasets Frontiers in Oncology. 2020 | Pubmed ID: 32232009 GlycoBIST: A System for Automatic Glycan Profiling of a Target Protein Using Milli-Bead Array in a Tip Current Protocols in Protein Science. Mar, 2020 | Pubmed ID: 32073758 Glycomic Signatures of Plasma IgG Improve Preoperative Prediction of the Invasiveness of Small Lung Nodules Molecules (Basel, Switzerland). Dec, 2019 | Pubmed ID: 31861777 LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections Molecules (Basel, Switzerland). Aug, 2019 | Pubmed ID: 31443278 Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling Analytical Chemistry. Sep, 2019 | Pubmed ID: 31405276 Wisteria Floribunda Agglutinin Staining for the Quantitative Assessment of Cardiac Fibrogenic Activity in a Mouse Model of Dilated Cardiomyopathy Laboratory Investigation; a Journal of Technical Methods and Pathology. Nov, 2019 | Pubmed ID: 31253865 An Improved Method for Cell Type-Selective Glycomic Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection International Journal of Molecular Sciences. Feb, 2019 | Pubmed ID: 30736315 Lectin Microarray Analyses Reveal Host Cell-specific Glycan Profiles of the Hemagglutinins of Influenza A Viruses Virology. Jan, 2019 | Pubmed ID: 30503907 Response to the Letter by Dr. Naoya Yamada, and Dr. Koichi Mizuta Regarding Our Manuscript: "Mac-2 Binding Protein Glycan Isomer (M2BPGi) is a New Serum Biomarker for Assessing Liver Fibrosis: More Than a Biomarker of Liver Fibrosis" Journal of Gastroenterology. Feb, 2019 | Pubmed ID: 30413874 Enhanced Azidolysis by the Formation of Stable Ser-His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant Journal of Applied Glycoscience. 2018 | Pubmed ID: 34354506 Current Technologies for Complex Glycoproteomics and Their Applications to Biology/Disease-Driven Glycoproteomics Journal of Proteome Research. Dec, 2018 | Pubmed ID: 30359034 Highly Sensitive Glycan Profiling of Hepatitis B Viral Particles and a Simple Method for Dane Particle Enrichment Analytical Chemistry. Sep, 2018 | Pubmed ID: 30074767 Mac-2 Binding Protein Glycan Isomer (M2BPGi) is a New Serum Biomarker for Assessing Liver Fibrosis: More Than a Biomarker of Liver Fibrosis Journal of Gastroenterology. Jul, 2018 | Pubmed ID: 29318378 Assessment of Tumor Characteristics Based on Glycoform Analysis of Membrane-tethered MUC1 Laboratory Investigation; a Journal of Technical Methods and Pathology. Sep, 2017 | Pubmed ID: 28581490 Serum Wisteria Floribunda Agglutinin-Positive Sialylated Mucin 1 As a Marker of Progenitor/Biliary Features in Hepatocellular Carcinoma Scientific Reports. Mar, 2017 | Pubmed ID: 28325920 A Standardized Method for Lectin Microarray-based Tissue Glycome Mapping Scientific Reports. Mar, 2017 | Pubmed ID: 28262709 Hepatic Stellate Cells Secreting WFA -M2BP: Its Role in Biological Interactions with Kupffer Cells Journal of Gastroenterology and Hepatology. Jul, 2017 | Pubmed ID: 28008658 Wisteria Floribunda Agglutinin-sialylated Mucin Core Polypeptide 1 is a Sensitive Biomarker for Biliary Tract Carcinoma and Intrahepatic Cholangiocarcinoma: a Multicenter Study Journal of Gastroenterology. Feb, 2017 | Pubmed ID: 27358229 Serum WFA -M2BP Levels for Evaluation of Early Stages of Liver Fibrosis in Patients with Chronic Hepatitis B Virus Infection Liver International : Official Journal of the International Association for the Study of the Liver. Jan, 2017 | Pubmed ID: 27300763 Alteration of Matrix Metalloproteinase-3 O-glycan Structure As a Biomarker for Disease Activity of Rheumatoid Arthritis Arthritis Research & Therapy. May, 2016 | Pubmed ID: 27209430 NMR Analysis on the Sialic Acid-binding Mechanism of an R-type Lectin Mutant by Natural Evolution-mimicry FEBS Letters. Jun, 2016 | Pubmed ID: 27172906 Influenza A Virus-Induced Expression of a GalNAc Transferase, GALNT3, Via MicroRNAs Is Required for Enhanced Viral Replication Journal of Virology. 02, 2016 | Pubmed ID: 26637460 A Novel Glycobiomarker, Wisteria Floribunda Agglutinin Macrophage Colony-stimulating Factor Receptor, for Predicting Carcinogenesis of Liver Cirrhosis International Journal of Cancer. Mar, 2016 | Pubmed ID: 26437001 Verification of WFA-Sialylated MUC1 As a Sensitive Biliary Biomarker for Human Biliary Tract Cancer Annals of Surgical Oncology. Feb, 2016 | Pubmed ID: 26416714 Lectin Microarray Technology Identifies Specific Lectins Related to Lymph Node Metastasis of Advanced Gastric Cancer Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. Apr, 2016 | Pubmed ID: 25840959 Lectin Microarray-Based Sero-Biomarker Verification Targeting Aberrant O-Linked Glycosylation on Mucin 1 Analytical Chemistry. Jul, 2015 | Pubmed ID: 26091356 Serum Wisteria Floribunda Agglutinin-Positive Mac-2 Binding Protein Values Predict the Development of Hepatocellular Carcinoma Among Patients with Chronic Hepatitis C After Sustained Virological Response PloS One. 2015 | Pubmed ID: 26070204 Development and Applications of the Lectin Microarray Topics in Current Chemistry. 2015 | Pubmed ID: 25821171 Clinicopathological Characteristics and Diagnostic Performance of Wisteria Floribunda Agglutinin Positive Mac-2-binding Protein As a Preoperative Serum Marker of Liver Fibrosis in Hepatocellular Carcinoma Journal of Gastroenterology. Nov, 2015 | Pubmed ID: 25773774 Wisteria Floribunda Agglutinin Positive Human Mac-2-binding Protein As a Predictor of Hepatocellular Carcinoma Development in Chronic Hepatitis C Patients Hepatology Research : the Official Journal of the Japan Society of Hepatology. Oct, 2015 | Pubmed ID: 25559682 LecT-Hepa Facilitates Estimating Treatment Outcome During Interferon Therapy in Chronic Hepatitis C Patients Clinical Proteomics. 2014 | Pubmed ID: 25593566 Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma Journal of Proteome Research. Nov, 2014 | Pubmed ID: 25244057 Differential Glycan Analysis of an Endogenous Glycoprotein: Toward Clinical Implementation--from Sample Pretreatment to Data Standardization Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 25117242 IgA Nephropathy Caused by Unusual Polymerization of IgA1 with Aberrant N-glycosylation in a Patient with Monoclonal Immunoglobulin Deposition Disease PloS One. 2014 | Pubmed ID: 24651839 Novel Glycobiomarker for Ovarian Cancer That Detects Clear Cell Carcinoma Journal of Proteome Research. Mar, 2014 | Pubmed ID: 24498956 Application of a Glycoproteomics-based Biomarker Development Method: Alteration in Glycan Structure on Colony Stimulating Factor 1 Receptor As a Possible Glycobiomarker Candidate for Evaluation of Liver Cirrhosis Journal of Proteome Research. Mar, 2014 | Pubmed ID: 24422531 Multilectin-assisted Fractionation for Improved Single-dot Tissue Glycome Profiling in Clinical Glycoproteomics Molecular BioSystems. Feb, 2014 | Pubmed ID: 24301485 Reconstruction of a Robust Glycodiagnostic Agent Supported by Multiple Lectin-assisted Glycan Profiling Proteomics. Clinical Applications. Oct, 2013 | Pubmed ID: 23640794 Glycoproteomics-based Cancer Marker Discovery Adopting Dual Enrichment with Wisteria Floribunda Agglutinin for High Specific Glyco-diagnosis of Cholangiocarcinoma Journal of Proteomics. Jun, 2013 | Pubmed ID: 23612463 Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-associated Liver Fibrosis and Hepatocellular Carcinoma Journal of Proteome Research. Jun, 2013 | Pubmed ID: 23586699 A Serum "sweet-doughnut" Protein Facilitates Fibrosis Evaluation and Therapy Assessment in Patients with Viral Hepatitis Scientific Reports. 2013 | Pubmed ID: 23323209 Directed Evolution of Lectins with Sugar-binding Specificity for 6-sulfo-galactose The Journal of Biological Chemistry. Jun, 2012 | Pubmed ID: 22493425 High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension Toward Alzheimer's Disease Biomarker Development International Journal of Alzheimer's Disease. 2011 | Pubmed ID: 21876827 LecT-Hepa: A Triplex Lectin-antibody Sandwich Immunoassay for Estimating the Progression Dynamics of Liver Fibrosis Assisted by a Bedside Clinical Chemistry Analyzer and an Automated Pretreatment Machine Clinica Chimica Acta; International Journal of Clinical Chemistry. Sep, 2011 | Pubmed ID: 21645500 Lectin-based Structural Glycomics: a Practical Approach to Complex Glycans Electrophoresis. May, 2011 | Pubmed ID: 21544837 Multilectin Assay for Detecting Fibrosis-specific Glyco-alteration by Means of Lectin Microarray Clinical Chemistry. Jan, 2011 | Pubmed ID: 21047982 A Versatile Technology for Cellular Glycomics Using Lectin Microarray Methods in Enzymology. 2010 | Pubmed ID: 20816480 Differential Glycan Profiling by Lectin Microarray Targeting Tissue Specimens Methods in Enzymology. 2010 | Pubmed ID: 20816479 Beta3GnT2 (B3GNT2), a Major Polylactosamine Synthase: Analysis of B3GNT2-deficient Mice Methods in Enzymology. 2010 | Pubmed ID: 20816167 Wisteria Floribunda Agglutinin-positive Mucin 1 is a Sensitive Biliary Marker for Human Cholangiocarcinoma Hepatology (Baltimore, Md.). Jul, 2010 | Pubmed ID: 20578261 A Strategy for Discovery of Cancer Glyco-biomarkers in Serum Using Newly Developed Technologies for Glycoproteomics The FEBS Journal. Jan, 2010 | Pubmed ID: 19919546 Mechanism by Which the Lectin Actinohivin Blocks HIV Infection of Target Cells Proceedings of the National Academy of Sciences of the United States of America. Sep, 2009 | Pubmed ID: 19717426 Engineering a Versatile Tandem Repeat-type Alpha2-6sialic Acid-binding Lectin Biochemical and Biophysical Research Communications. Jun, 2009 | Pubmed ID: 19393618 Strategy for Glycoproteomics: Identification of Glyco-alteration Using Multiple Glycan Profiling Tools Journal of Proteome Research. Mar, 2009 | Pubmed ID: 19178301 Focused Differential Glycan Analysis with the Platform Antibody-assisted Lectin Profiling for Glycan-related Biomarker Verification Molecular & Cellular Proteomics : MCP. Jan, 2009 | Pubmed ID: 18697734 Optimization of Evanescent-field Fluorescence-assisted Lectin Microarray for High-sensitivity Detection of Monovalent Oligosaccharides and Glycoproteins Proteomics. Aug, 2008 | Pubmed ID: 18615430 Development of an All-in-one Technology for Glycan Profiling Targeting Formalin-embedded Tissue Sections Biochemical and Biophysical Research Communications. May, 2008 | Pubmed ID: 18375199 Engineering of Mucin-type Human Glycoproteins in Yeast Cells Proceedings of the National Academy of Sciences of the United States of America. Mar, 2008 | Pubmed ID: 18296643 Polylactosamine on Glycoproteins Influences Basal Levels of Lymphocyte and Macrophage Activation Proceedings of the National Academy of Sciences of the United States of America. Oct, 2007 | Pubmed ID: 17890318 A Novel Strategy for Mammalian Cell Surface Glycome Profiling Using Lectin Microarray Glycobiology. Oct, 2007 | Pubmed ID: 17693441 Tailoring a Novel Sialic Acid-binding Lectin from a Ricin-B Chain-like Galactose-binding Protein by Natural Evolution-mimicry Journal of Biochemistry. Mar, 2007 | Pubmed ID: 17234683 Functional Glycosylation of Human Podoplanin: Glycan Structure of Platelet Aggregation-inducing Factor FEBS Letters. Jan, 2007 | Pubmed ID: 17222411 Inhibition of Tumor Cell-induced Platelet Aggregation Using a Novel Anti-podoplanin Antibody Reacting with Its Platelet-aggregation-stimulating Domain Biochemical and Biophysical Research Communications. Nov, 2006 | Pubmed ID: 16979138 A Combined Strategy for Glycan Profiling: a Model Study with Pyridylaminated Oligosaccharides Journal of Biochemistry. Sep, 2006 | Pubmed ID: 16861248 The Family 42 Carbohydrate-binding Module of Family 54 Alpha-L-arabinofuranosidase Specifically Binds the Arabinofuranose Side Chain of Hemicellulose The Biochemical Journal. Nov, 2006 | Pubmed ID: 16846393 Evidence That Agaricus Bisporus Agglutinin (ABA) Has Dual Sugar-binding Specificity Biochemical and Biophysical Research Communications. Aug, 2006 | Pubmed ID: 16824489 Characterization of an Exo-beta-1,3-galactanase from Clostridium Thermocellum Applied and Environmental Microbiology. May, 2006 | Pubmed ID: 16672498 Overexpression, Purification and Crystallization of Tyrosyl-tRNA Synthetase from the Hyperthermophilic Archaeon Aeropyrum Pernix K1 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Nov, 2005 | Pubmed ID: 16511219 Evanescent-field Fluorescence-assisted Lectin Microarray: a New Strategy for Glycan Profiling Nature Methods. Nov, 2005 | Pubmed ID: 16278656 A Hepatitis C Virus (HCV) Internal Ribosome Entry Site (IRES) Domain III-IV-targeted Aptamer Inhibits Translation by Binding to an Apical Loop of Domain IIId Nucleic Acids Research. 2005 | Pubmed ID: 15681618 Structure-inhibition Analysis of RNA Aptamers That Bind to HCV IRES Nucleic Acids Research. Supplement (2001). 2003 | Pubmed ID: 14510495 RNA Aptamers Targeted to Domain II of Hepatitis C Virus IRES That Bind to Its Apical Loop Region Journal of Biochemistry. Mar, 2003 | Pubmed ID: 12761160 In Vitro Selection of RNA Aptamers That Bind to Domain II of HCV IRES Nucleic Acids Research. Supplement (2001). 2002 | Pubmed ID: 12903207 Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System Sayaka Fuseya1, Ayaka Ono1, Hiromi Ootani1, Saho Mizukado1, Tomomi Obayashi2, Nana Tanaka2, Hiroko Shimazaki1,2, Kenji Kajiyama2, Moe Ashitomi2, Shiori Yasuda2, Takenori Miyabe2, Kazuhiro Nakamura2, Osamu Segawa2, Kazumi Sawakami2, Atsushi Kuno1 1Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, 2Precision System Science Co., Ltd. JoVE 66571 생화학
Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System Sayaka Fuseya1, Ayaka Ono1, Hiromi Ootani1, Saho Mizukado1, Tomomi Obayashi2, Nana Tanaka2, Hiroko Shimazaki1,2, Kenji Kajiyama2, Moe Ashitomi2, Shiori Yasuda2, Takenori Miyabe2, Kazuhiro Nakamura2, Osamu Segawa2, Kazumi Sawakami2, Atsushi Kuno1 1Molecular & Cellular Glycoproteomics Research Group, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, 2Precision System Science Co., Ltd. JoVE 66571 생화학