Adipose Tissue-Derived Stem Cell Isolation and Characterization
April 7th, 2023Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats (Video) | JoVE
02:06 min
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Murine Adipose-Derived Mesenchymal Cell Isolation, Maintenance and Characterization (Video) | JoVE
03:12 min
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Immunofluorescent Detection of Adipose-Derived Stem Cell Expression Markers (Video) | JoVE
01:39 min
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Aymé Oliva Cárdenas1,2, Blanca C. Zamora-Rodríguez2, Kevin A. Batalla-García2, Alejandro Ávalos-Rodríguez3, Alejandra Contreras-Ramos4, Clara Ortega-Camarillo2
1Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, 2Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI, Instituto Mexicano de Seguro Social, 3Department of Agricultural and Animal Production, Division of Biological and Health Science, Universidad Autónoma Metropolitana-Xochimilco, 4Molecular Biology Research Laboratory, Center for Congenital Malformations, Children Hospital of Mexico Federico Gomez (HIMFG)
Summary
이 프로토콜은 Sprague Dawley 쥐에서 지방 조직 유래 중간엽 줄기 세포(MSC)를 분리하고 식별하는 방법론을 설명합니다.