Viability Assay of <em>Trichoderma stromaticum</em> Conidia Inside Human Peripheral Blood Mononuclear-Derived Macrophages

Uener Ribeiro dos Santos; Julyanna Oliveira de Castro; Melissa Erc&#237;lia Santos Matos; Giulia De Bonis; Jane Lima dos Santos

doi:10.3791/65231

Fungal Clearance Efficiency of Macrophages

October 20, 2023

Author Spotlight: Unraveling the Interplay Between Trichoderma stromaticum and the Mammalian Immune System

01:30 min

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Culturing Trichoderma stromaticum and Obtaining Conidia for Phagocytosis Assay

01:01 min

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Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Human Blood and Differentiation of PBMCs into Macrophages for Phagocytosis Assay

01:03 min

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Phagocytosis Kinetics and Trichoderma stromaticum Conidial Viability Assay Using Human Peripheral Blood Mononuclear-Derived Macrophages

03:58 min

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Uener Ribeiro dos Santos*¹, Julyanna Oliveira de Castro*¹, Melissa Ercília Santos Matos, Giulia De Bonis, Jane Lima dos Santos

¹Laboratory of Immunobiology – Department of Biological Sciences,State University of Santa Cruz – UESC, ²Università Degli Studi di Genova

Summary

The technique involving the phagocytosis of fungal conidia by macrophages is widely used for studies evaluating the modulation of the immune responses against fungi. The purpose of this manuscript is to present a method for evaluating the phagocytosis and clearance abilities of human peripheral blood mononuclear-derived macrophages stimulated with Trichoderma stromaticum conidia.