A Nanopore-Based Genomic Approach to Control Rabies in Low-Income Countries

August 18, 2023

使用纳米孔技术快速表征狂犬病病毒的全基因组测序

狂犬病病毒（RABV）全基因组测序的样品制备、RNA 提取、PCR 纯化和定量

狂犬病病毒（RABV）基因组的测序、活体和离线碱基检出、分析和解释

Criselda Bautista², Gurdeep Jaswant^3,4,5, Hollie French⁶, Kathryn Campbell, Rowan Durrant, Robert Gifford⁶, Grace S. N. Kia⁸, Brian Ogoti⁹, Katie Hampson, Kirstyn Brunker⁶

¹School of Biodiversity, One Health & Veterinary Medicine,University of Glasgow, ²Research Institute for Tropical Medicine, ³University of Nairobi, Institute of Tropical and Infectious Diseases, ⁴Tanzania Industrial Research Development Organization, ⁵Ifakara Health Institute, ⁶MRC-University of Glasgow Centre for Virus Research, ⁷Department of Veterinary Public health,Ahmadu Bello University, ⁸African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology,Ahmadu Bello University, ⁹University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis,University of Nairobi

Summary

在这里，我们提出了一种使用纳米孔技术表征狂犬病病毒（RABV）基因组的快速且具有成本效益的工作流程。该工作流程旨在支持地方层面的基因组学监测，提供有关循环RABV谱系及其在区域系统发育中的位置的信息，以指导狂犬病控制措施。

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