Liver Transplantation in Mouse and Analyzing Allograft Patency

September 22, 2023

Enhancing Transplantation Research Through MicroCT Angiography in Murine Models (Video) | JoVE

01:41 min

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Midline Laparotomy Surgery in Donor Mice for Orthotopic Liver Transplantation (Video) | JoVE

02:41 min

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Midline Laparotomy Surgery in Recipient Mice for Orthotopic Liver Transplantation (Video) | JoVE

02:33 min

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Mouse MicroCT Angiography Imaging to Evaluate Allograft Patency and Durability of Anastomoses After Liver Transplantation (Video) | JoVE

02:39 min

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Qiang Zeng, Doug A. Gouchoe³, Mahboubeh Nabavinia, Yong Gyu Lee, Xi Wang, Terri A. Shaffer, Mitchel R. Stacy^5,6, Blake R. Peterson⁸, Bryan A. Whitson⁹, Christopher Breuer⁴, Sylvester M. Black¹⁰

¹Department of Surgery,Nationwide Children’s Hospital, ²COPPER Laboratory,The Ohio State University, ³88th Surgical Operation Operations Squadron,WPAFB, ⁴Center for Regenerative Medicine,The Research Institute at Nationwide Children’s Hospital, ⁵Division of Vascular Diseases and Surgery,The Ohio State University College of Medicine, ⁶Interdisciplinary Biophysics Graduate Program,The Ohio State University, ⁷Division of Medicinal Chemistry and Pharmacognosy,The Ohio State University College of Pharmacy, ⁸The Ohio State University Comprehensive Cancer Center, ⁹Division of Cardiac Surgery, Department of Surgery,The Ohio State Wexner Medical Center, ¹⁰Division of Transplantation, Department of Surgery,The Ohio State Wexner Medical Center

Summary

In this protocol, we discuss the implementation of a model of successful orthotopic liver transplantation (OLT) in mice. Additionally, adjuvants to further analyze allograft patency after successful OLT in a mouse are discussed as well, specifically utilizing microcomputed tomography (microCT) scans.