Analyzing Mitochondrial Function in a <em>Drosophila melanogaster</em> PINK1<sup>B9</sup>-Null Mutant Using High-resolution Respirometry

Paula Michelotti; T&#226;mie Duarte; Cristiane L. Dalla Corte

doi:10.3791/65664

Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

November 10, 2023

Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster (Video) | JoVE

02:34 min

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Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster (Video) | JoVE

01:38 min

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Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE

05:12 min

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Paula Michelotti*^1,2, Tâmie Duarte*^1,2, Cristiane L. Dalla Corte²

¹Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences,Federal University of Santa Maria, ²Graduate Program in Biological Sciences: Toxicological Biochemistry,Federal University of Santa Maria

Summary

Here we present a high-resolution respirometry protocol to analyze bioenergetics in PINK1^B9-null mutant fruit flies. The method uses the Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocol.

Analyzing Mitochondrial Physiology and Bioenergetics in Fruit Flies

Exploring Mitochondrial Function and Chemical Toxicity Using Drosophila melanogaster (Video) | JoVE

Calibrating Polarographic Oxygen Sensors for High-Resolution Respirometry Analysis in Drosophila melanogaster (Video) | JoVE

Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE

Summary

Article