Cost-Efficient Transcriptomic-Based Drug Screening

Jacqueline Leidner; Heidi Theis; Michael Kraut; Alice Ragogna; Marc Beyer; Joachim Schultze; Jonas Schulte-Schrepping; Caterina Carraro; Lorenzo Bonaguro

doi:10.3791/65930

Transcriptome Profiling for High-Throughput Drug Screening

February 23, 2024

Cost-Effective Transcriptomic Drug Screening â€” Unlocking New Targets (Video) | JoVE

01:10 min

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Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE

01:56 min

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CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing (Video) | JoVE

02:34 min

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MRNA Sequencing to Understand the Cellular Effect of Drug Treatment (Video) | JoVE

01:11 min

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Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

Summary

This protocol describes a workflow from ex vivo or in vitro cell cultures to transcriptomic data pre-processing for cost-effective transcriptome-based drug screening.

Transcriptome Profiling for High-Throughput Drug Screening

Cost-Effective Transcriptomic Drug Screening â€” Unlocking New Targets (Video) | JoVE

Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing (Video) | JoVE

CDNA Library Preparation from the Drug Treated-Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing (Video) | JoVE

MRNA Sequencing to Understand the Cellular Effect of Drug Treatment (Video) | JoVE

Summary

Article