Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells <em>via</em> Transendothelial Electrical Resistance Detection

Fuhan Fan; Hong Jiang; Ya Hou; Yi Zhang; Qipeng Zhao; Yong Zeng; Xianli Meng; Xiaobo Wang

doi:10.3791/65938

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

September 29, 2023

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

01:19 min

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Culture of bEnd.3 Cells and Cell Viability After CoCl2 Treatment (Video) | JoVE

02:32 min

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In Vitro Blood-Brain Barrier Model Assembly for Measurement of TEER (Video) | JoVE

03:39 min

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Barrier Destruction and Statistical Analysis After TEER Measurement in an In Vitro Blood-Brain Barrier Model (Video) | JoVE

01:38 min

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Fuhan Fan, Hong Jiang, Ya Hou, Yi Zhang^2,3, Qipeng Zhao, Yong Zeng², Xianli Meng^2,5, Xiaobo Wang

¹School of Pharmacy/School of Modern Chinese Medicine Industry,Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy,Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline,Chengdu University of Traditional Chinese Medicine

Summary

This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance.