An All-Human Hepatic Culture System for Drug Development Applications

Justin J. Odanga; Elena Gianulis; Lindsey Whaley; Edward L. LeCluyse; Sharon Presnell; Jessica R. Weaver

doi:10.3791/65992

A Physiologically-Relevant All-Human 2D+ Hepatic System for Drug Discovery

October 20, 2023

Developing a Simple and Robust Hepatic Model for Pharmacological and Toxicological Applications (Video) | JoVE

01:11 min

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Media Preparation for Setting Up the All-Human 2D+ Hepatic Culture System (Video) | JoVE

01:12 min

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Plating Human Feeder Cells for Setting Up the All-Human 2D+ Hepatic Culture System (Video) | JoVE

01:43 min

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Plating Primary Human Hepatocytes for Setting Up the All-Human 2D+ Hepatic Culture System (Video) | JoVE

03:30 min

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Justin J. Odanga, Elena Gianulis, Lindsey Whaley, Edward L. LeCluyse, Sharon Presnell, Jessica R. Weaver

¹Institute of Regenerative Medicine,LifeNet Health, ²Biologics Division,LifeNet Health, ³LifeSciences Division,LifeNet Health, ⁴Research and Development,LifeSciences, LifeNet Health

Summary

Recent technical advances enabled the scaled production of an in vitro platform for drug metabolism and toxicity applications. An all-human 2D+ hepatic system (TV2D+) provides physiologically relevant results using traditional two-dimensional culture methods. This protocol will support end-users in the setup, maintenance, and application of the system.