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Fabrication of Streptavidin Affinity Grids for Cryo-EM Structural Studies

December 29th, 2023
1, 2,3, 4,5, 6, 1,2,6, 6, 1,2,3,6
1Department of Molecular and Cell Biology, University of California, Berkeley, 2California Institute for Quantitative Biology (QB3), University of California, Berkeley, 3Howard Hughes Medical Institute, University of California, Berkeley, 4Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital, University of Cologne, 5Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD), University of Cologne, 6Molecular Biophysics and Integrative Bio-Imaging Division, Lawrence Berkeley National Laboratory

Summary
Automatic Translation

Un protocole étape par étape pour la fabrication de grilles d’affinité de la streptavidine est fourni pour une utilisation dans les études structurales d’échantillons macromoléculaires difficiles par cryo-microscopie électronique.

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