Preliminary Validation of Stereotaxic Injection Coordinates via Cryosectioning

Xiaoxin Zhou; Wanbing Dai; Jie Zhou; Yizhe Zhang; Xiao Zhang; Sihan Chen; Xiaoyu Sun

doi:10.3791/66262

Optimizing Stereotaxic Microinjection Accuracy in Mice

July 19, 2024

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Targeted Brain Region Microinjection of Bromophenol Dye in Mice

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Brain Tissue Harvesting, Cryosectioning, and Imaging Post Stereotaxic Surgery in Mice

02:42 min

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Xiaoxin Zhou*^1,2,3, Wanbing Dai*^1,2, Jie Zhou*^1,2, Yizhe Zhang², Xiao Zhang², Sihan Chen², Xiaoyu Sun²

¹Department of Anesthesiology, Renji Hospital,Shanghai Jiaotong University, School of Medicine, ²Key Laboratory of Anesthesiology (Shanghai Jiaotong University), Ministry of Education, ³Department of Anesthesiology, Shanghai General Hospital,Shanghai Jiaotong University

Summary

The present protocol describes a practical strategy to expedite the verification step of stereotaxic injection coordinates before conducting viral tracing using dyes and frozen sections.

Optimizing Stereotaxic Microinjection Accuracy in Mice

Author Spotlight: Accelerating Cognitive Impairment Research in Mice Through Stereotaxic Injection and Cost-Effective Dyes

Targeted Brain Region Microinjection of Bromophenol Dye in Mice

Brain Tissue Harvesting, Cryosectioning, and Imaging Post Stereotaxic Surgery in Mice

Summary

Article