FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency

February 02, 2024

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

02:29 min

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Lu-Ping Zhang, Yong-Hong Nie, Tuo Tang, Ai-Xue Zheng, Xian Hong, Tao Wang

¹Laboratory of Protein Structure and Function, Institute of Medicine and Pharmacy,Qiqihar Medical University

Summary

This protocol describes an extrachromosomal nonhomologous end joining (NHEJ) assay and homologous recombination (HR) assay to quantify the efficiency of NHEJ and HR in HEK-293T cells.

FACS Analysis of GFP and mCherry Reporter Cells to Evaluate NHEJ & HR Efficiency

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

Summary

Article