1Institutes of Biomedical Sciences,Fudan University, 2Clinical Center of Biotherapy at Zhongshan Hospital,Fudan University, 3Department of Oncology and Bio-therapeutic Center, Shenzhen Third People’s Hospital,Southern University of Science and Technology, 4Shanghai Public Health Clinical Center,Fudan University
Here we present a protocol for the generation and functional verification of hypoxia-sensitive chimeric antigen receptor (CAR)-T cells. This protocol presents the lentivirus-based generation of hypoxia-sensitive CAR-T cells and their characterization, including the validation of hypoxia-dependent CAR expression and selective cytotoxicity.
