Xiamen University View Institution's Website 5 articles published in JoVE Biology Primary Culture of Porcine Retinal Pigment Epithelial Cells Feng Wen*1,2,3, Yanzi Wang*1,2,3, Danxue He1,2,3, Chunyan Liao1,2,3, Weijie Ouyang1,2,3, Zuguo Liu1,2,3, Wei Li1,2,3, Yi Liao1,2,3 1Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, School of Medicine, Xiamen University, 2Department of Ophthalmology, Xiang'an Hospital of Xiamen University, School of Medicine, Xiamen University, 3Xiamen University Affiliated Xiamen Eye Center, School of Medicine, Xiamen University Here, an easy-to-follow method to culture primary porcine retinal pigment epithelial cells in vitro is presented. Immunology and Infection Optimization of the Cuff Technique for Murine Heart Transplantation Yunhan Ma*1,2, Baiyi Xie*1,2, Helong Dai*3,4,5, Chenxi Wang6, Shujiao Liu6, Tianshu Lan7, Shuangyue Xu1,2, Guoliang Yan1,2,6, Zhongquan Qi1,2,8 1Organ Transplantation Institute, School of Medicine, Xiamen University, 2Fujian Key Laboratory of Organ and Tissue Regeneration, 3Department of Kidney Transplantation, Center of Organ Transplantation, The Second Xiangya Hospital of Central South University, 4Clinical Research Center for Organ Transplantation in Hunan Province, 5Clinical Immunology Center, Central South University, 6School of Medicine, Xiamen University, 7Xiamen Medical College, 8Medical College, Guangxi University We introduce an inner tube approach to the cuff technique for mouse cervical heterotopic heart transplantation to help evert the vessel over the cuff. We found that cooperation between two experienced surgeons markedly shortens the operation time. Biochemistry Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers Huijuan You*1, Shimin Le*2,3, Hu Chen2,4, Linyan Qin1, Jie Yan2,4,5 1School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, 2Mechanobiology Institute, National University of Singapore, 3Department of Physics, National University of Singapore, 4Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, Xiamen University, 5Center for Bioimaging Sciences, National University of Singapore A single-molecule magnetic tweezers platform to manipulate G-quadruplexes is reported, which allows for the study of G4 stability and regulation by various proteins. Engineering Characterization of Full Set Material Constants and Their Temperature Dependence for Piezoelectric Materials Using Resonant Ultrasound Spectroscopy Liguo Tang1, Wenwu Cao2 1Key Laboratory of Underwater Acoustic Communication and Marine Information Technology, Xiamen University, 2Department of Mathematics and Material Research Institute, The Pennsylvania State University This protocol describes the procedure of measuring the temperature dependence of the full set material constants of piezoelectric materials using resonant ultrasound spectroscopy (RUS). Immunology and Infection Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites Sittiporn Pattaradilokrat1, Jian Li1,2, Xin-zhuan Su1 1National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2School of Life Science, Xiamen University Genetic crosses of rodent malaria parasites are performed by feeding two genetically distinct parasites to mosquitoes. Recombinant progeny are cloned from mouse blood after allowing mosquitoes to bite infected mice. This video shows how to produce genetic crosses of Plasmodium yoelii and is applicable to other rodent malaria parasites.