Guangdong Medical University 3 articles published in JoVE Medicine Plasma Polishing as a New Polishing Option to Reduce the Surface Roughness of Porous Titanium Alloy for 3D Printing Zhiwei Lin*1,2, Lincong Luo*3, Dongxin Lin*4, Yuping Deng4,5, Yang Yang4, Xuecheng Huang6, Tingrui Wu1, Wenhua Huang1,2,4 1Orthopedic Center, Affiliated Hospital of Guangdong Medical University, 2The School of Basic Medical Sciences, Guangdong Medical University, 3The School of Basic Medical Sciences, Fujian Medical University, 4Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, 5Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, 6Shenzhen Hospital of Guangzhou University of Chinese Medicine (Futian) Plasma polishing is a promising surface processing technology, especially suitable for 3D printing of porous titanium alloy workpieces. It can remove semi-molten powders and ablative oxide layers, thereby effectively reducing surface roughness and improving surface quality. Biology Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs Yanli Zhao1,2,3, Chengjiang Zhao4, David K.C. Cooper5, Ying Lu2, Kewang Luo6, Huiyun Wang3, Pengfei Chen3, Changchun Zeng3, Shaodong Luan1, Lisha Mou2, Hanchao Gao1,2,3 1Department of Nephrology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology, Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, 6 An effective enzymatic method for isolation of primary porcine aortic endothelial cells (pAECs) from miniature pigs is described. The isolated primary pAECs can be used to investigate the immune and coagulation response in xenotransplantation. Cancer Research Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles Baozhen Zhang*1, Mingbin Zheng*2,3, Lintao Cai2, Xiujun Fan1 1Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), 2Guangdong Key Laboratory of Nanomedicine, CAS Key Lab for Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS), 3Department of Chemistry, Guangdong Medical University Here, we present a protocol for the synthesis of placental chondroitin sulfate A binding peptide (plCSA-BP)-conjugated lipid-polymer nanoparticles via single-step sonication and bioconjugate techniques. These particles constitute a novel tool for the targeted delivery of therapeutics to most human tumors and placental trophoblasts to treat cancers and placental disorders.