Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education 3 articles published in JoVE Medicine Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice Chujie Zhou1,2, Xingyuan Sun1,2, Junli Chang1,2, Junjie Tong1,2, Peng Zhao1,2, Suxia Guo1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education The protocol provides a detailed standardization process of decoction formula and gavage technique with Yiqi Jiedu decoction in the osteosarcoma mouse model as an example. It describes animal protection and improves reliability of research data, providing effective strategies for investigating therapeutic efficacy and molecular mechanisms of decoction formulas in vivo. Cancer Research Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models Junli Chang*1,2, Fulai Zhao*1,2, Xingyuan Sun1,2, Xiaoping Ma1,2, Wenlan Zhi1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions. Medicine Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments Yongjian Zhao1,2,3, Qiang Wang1,2,3, Shufen Liu1,2,3, Yongjun Wang1,2,3,4, Bing Shu1,2,3, Dongfeng Zhao1,2,3 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Spine Institute, Shanghai University of Traditional Chinese Medicine, 3Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 4School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro experiments.
