Chongqing Medical University 2 articles published in JoVE Developmental Biology Generation, Maintenance, and Identification of Germ-Free Zebrafish Models from Larvae to Juvenile Stages Pan-Pan Jia1, Xiang-Ru Liu1, Ming-Fei Wu1, Yan Li1, Lan-Chen Zhang1, De-Sheng Pei1 1School of Public Health, Chongqing Medical University This protocol outlines the primary steps for obtaining germ-free (GF) fish embryos and maintaining them from larvae until the juvenile stage, including sampling and detecting their sterile status. The use of GF models with infection is important for understanding the role of microbes in host health. Immunology and Infection Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis Yan Zhang*1, Xingxing Su*2, Lisha Wang2, Zhengliang Yue2, Qiao Liu3, Ling Ran2, Shun Lei2, Jianjun Hu2, Lifan Xu2, Lilin Ye2, Ping Ji4, Guimei Li1, Qizhao Huang5, Shuqiong Wen4 1College of Veterinary Medicine, Qingdao Agricultural University, 2Institute of Immunology, Third Military Medical University, 3Institute of Cancer, Xinqiao Hospital, Third Military Medical University, 4Stomatological Hospital of Chongqing Medical University, 5Institute of life science, Chongqing Medical University The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T cells.