Ben-Gurion University View Institution's Website 5 articles published in JoVE Biology Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay Tomer Eli Ben Yosef1, Raz Zarivach1, Arie Moran2 1Department of Life Sciences and National Institute for Biotechnology in the Negev, Ben-Gurion University, 2Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University Zinc transport has proven challenging to measure due to the weak causal links to protein function and the low temporal resolution. This protocol describes a method for monitoring, with high temporal resolution, Zn2+ extrusion from living cells by utilizing a Zn2+ sensitive fluorescent dye, thus providing a direct measure of Zn2+ efflux. Chemistry Exploring the Radical Nature of a Carbon Surface by Electron Paramagnetic Resonance and a Calibrated Gas Flow Uri Green1,2, Yulia Shenberger3, Zeev Aizenshtat1, Haim Cohen2,4, Sharon Ruthstein3 1Chemistry Institute, The Hebrew University of Jerusalem, 2Biological Chemistry Department, Ariel University, 3Chemistry Department, Faculty of Exact Science, Bar Ilan University, 4Chemistry Department, Ben-Gurion University Stable radicals that are present in carbon substrates interact with paramagnetic oxygen through a Heisenberg spin exchange. This interaction can be significantly reduced under STP conditions by flowing a diamagnetic gas over the carbon system. This manuscript describes a simple method to characterize the nature of those radicals. Neuroscience One Dimensional Turing-Like Handshake Test for Motor Intelligence Amir Karniel1, Guy Avraham1, Bat-Chen Peles1, Shelly Levy-Tzedek1, Ilana Nisky1 1Biomedical Engineering, Ben-Gurion University We present a Turing-like Handshake test administered through a telerobotic system in which the interrogator is holding a robotic stylus and interacting with another party (human or artificial). We use a forced choice method, and extract a measure for the similarity of the artificial model to a human handshake. Biology Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41 Natalie Zeytuni1, Raz Zarivach1 1Department of Life Sciences and National Institute for Biotechnology in the Negev, Ben-Gurion University MamA is a unique Magnetosome associated protein which was shown to be involved in magnetosome activation. Here we present the purification protocol of MamA deletion mutant (MamAΔ41) from M. magneticum AMB-1. Biology Aortic Ring Assay Keren Bellacen1, Eli C. Lewis1 1Department Clinical Biochemistry, Ben-Gurion University Angiogenesis, the sprouting of blood vessels from pre-existing vasculature, is associated with both natural and pathological processes. Here we demonstrate an aortic ring assay that allows angiogenic potentiators and inhibitors to be directly added to aortic rings in culture. Sprouting and neovessel outgrowth can be determined by inspecting the aortic rings over a period of 6-12 days.