National Institute of Information and Communications Technology 4 articles published in JoVE Neuroscience Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI Hui Ming Khoo1, Yuya Fujita1, Naoki Tani1, Tetsuya Shimokawa2, Natalja Zazubovits3, Satoru Oshino1, Jean Gotman3, Haruhiko Kishima1 1Department of Neurosurgery, Osaka University Graduate School of Medicine, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3Montreal Neurological Institute and Hospital, McGill University This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products. Behavior Tactile Semiautomatic Passive-Finger Angle Stimulator (TSPAS) Wu Wang1, Jiajia Yang2,4, Yinghua Yu2,3,4, Qiong Wu5,2, Satoshi Takahashi2, Yoshimichi Ejima2, Jinglong Wu6,2 1Cognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama University, 2Cognitive Neuroscience Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3Center for Information and Neural Networks, National Institute of Information and Communications Technology, 4Section on Functional Imaging Methods, National Institute of Mental Health, 5School of Education, Suzhou University of Science and Technology, 6Beijing Institute of Technology Presented is the tactile semiautomated passive-finger angle stimulator TSPAS, a new way to assess tactile spatial acuity and tactile angle discrimination using a computer-controlled tactile stimulus system that applies raised angle stimuli to a subject's passive fingerpad, while controlling for movement speed, distance, and contact duration. Biology High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon Atsushi Matsuda1,2, Takako Koujin1, Lothar Schermelleh3, Tokuko Haraguchi1,2, Yasushi Hiraoka1,2 1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon. Neuroscience Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes Naoki Ihara1, Yuji Ikegaya1,2, Haruki Takeuchi1,3 1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST) Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.