Waseda University 10 articles published in JoVE Behavior Virtual Hand with Ambiguous Movement between the Self and Other Origin: Sense of Ownership and 'Other-Produced' Agency Mai Minoura1, Kei Kojima1, Shuusaku Nomura2, Yuta Nishiyama2, Takashi Kawai1, Yukio-Pegio Gunji1 1Department of Intermedia, Art and Science, School of Fundamental Science and Technology, Waseda University, 2Human Informatics Group, Graduate School of Information and Management Systems Engineering, Nagaoka University of Technology While previous research on bodily self-consciousness assumed that self- and other-origin movements were perceptually distinguishable, this protocol allows them to be ambiguous on a virtual hand with unintentional slight movements. This enables us to observe one’s experience formed by SoO and other-produced SoA, rather than the absence of SoA. Chemistry Organic Structure-directing Agent-free Synthesis for *BEA-type Zeolite Membrane Motomu Sakai1, Noriyuki Yasuda2, Yuto Tsuzuki2, Masahiko Matsukata1,2,3 1Research Organization for Nano & Life Innovation, Waseda University, 2Department of Applied Chemistry, Waseda University, 3Advanced Research Institute for Science and Engineering, Waseda University A *BEA seed crystal was loaded on a porous α-Al2O3 support by the dip-coating method, and hydrothermally grown without using an organic structure-directing agent. A *BEA-type zeolite membrane having very few defects was successfully prepared by the secondary growth method. Immunology and Infection Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes Naoyoshi Sakitani*1, Takahiro Maekawa*1, Kumiko Saitou1,2, Katsuhiko Suzuki3, Shuhei Murase1,4, Masakuni Tokunaga1, Daisuke Yoshino5, Keisuke Sawada6, Atsushi Takashima7, Motoshi Nagao1, Toru Ogata1, Yasuhiro Sawada1,8 1Department of Rehabilitation for Motor Functions, National Rehabilitation Center for Persons with Disabilities, 2Graduate School of Sport Sciences, Waseda University, 3Faculty of Sport Sciences, Waseda University, 4Department of Orthopaedic Surgery, Graduate School of Medicine, The University of Tokyo, 5Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6University of Cincinnati College of Medicine, 7Department of Assistive Technology, National Rehabilitation Center for Persons with Disabilities, 8Department of Clinical Research, National Rehabilitation Center for Persons with Disabilities Here we describe the protocols for applying defined mechanical loads to mouse calves and for monitoring the concomitant intramuscular pressure changes. The experimental systems that we have developed can be useful for investigating the mechanism behind the beneficial effects of physical exercise and massage. Immunology and Infection Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles Masamune Morita1, Yuri Ota1,2, Kaoru Katoh1, Naohiro Noda1,2 1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2Department of Life Science and Medical Bioscience, Waseda University We demonstrate single-cell culture of bacteria inside giant vesicles (GVs). GVs containing bacterial cells were prepared by the droplet transfer method and were immobilized on a supported membrane on a glass substrate for direct observation of bacterial growth. This approach may also be adaptable to other cells. Behavior Observing the Transformation of Bodily Self-consciousness in the Squeeze-machine Experiment Mai Minoura1, Iori Tani2, Takahiro Ishii3, Yukio- Pegio Gunji1 1Department of Intermedia Art and Science, Waseda University, 2Research Center for Kansei Value Creation, School of Science and Technology, Kwansei Gakuin University, 3Department of Occupational Therapy, Teikyo University of Science The squeeze machine, a V-shaped device that provides pressure along the whole body is used in therapy especially for people with autism. Here, we focus on its possible use in experiments to capture the typical transformations of bodily self-consciousness with peri-personal space measurement. Medicine A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device Kazuhiro Ogai1, Masakazu Fukuoka2, Kei-ichiro Kitamura3, Kiyoshi Uchide4, Tetsu Nemoto3 1Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Advanced Research Center for Human Sciences, Waseda University, 3Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 4Asanogawa General Hospital Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test. Engineering Fabrication of White Light-emitting Electrochemical Cells with Stable Emission from Exciplexes Soichi Uchida1, Daisuke Takizawa2, Satoru Ikeda1, Hironori Takeuchi3, Suzushi Nishimura1, Hiroyuki Nishide2, Yoshinori Nishikitani2 1Specialty Chemicals & Materials Company, JX Nippon Oil & Energy Corporation, 2Department of Advanced Science and Engineering, Waseda University, 3Central Technical Research Laboratory, JX Nippon Oil & Energy Corporation The authors present a method for fabricating stable white-light-emitting electrochemical cells utilizing emission from exciplexes formed between a blue-emitting fluorene polymer and aromatic amines. Engineering Detection and Recovery of Palladium, Gold and Cobalt Metals from the Urban Mine Using Novel Sensors/Adsorbents Designated with Nanoscale Wagon-wheel-shaped Pores Sherif A. El-Safty1,2, Mohamed A. Shenashen1, Masaru Sakai3, Emad Elshehy1, Kohmei Halada1 1National Institute for Materials Science, Japan, 2Graduate School for Advanced Science and Engineering, Waseda University, 3Center for Research in Isotopes and Environmental Dynamics, Tsukuba University Because of the importance and extensive use of palladium, gold and cobalt metals in high-technology equipment, their recovery and recycling constitute an important industrial challenge. The metal recovery system described herein is a simple, low-cost means for the effective detection, removal, and recovery of these metals from the urban mine. Bioengineering Photopatterning Proteins and Cells in Aqueous Environment Using TiO2 Photocatalysis Hideaki Yamamoto1,2, Takanori Demura3, Kohei Sekine3, Sho Kono3, Michio Niwano2,4, Ayumi Hirano-Iwata2,5, Takashi Tanii6 1Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 2CREST, Japan Science and Technology Agency, 3School of Fundamental Science and Engineering, Waseda University, 4Research Institute of Electrical Communication, Tohoku University, 5Graduate School of Biomedical Engineering, Tohoku University, 6Faculty of Science and Engineering, Waseda University We describe a protocol for modifying cell affinity of a scaffold surface in aqueous environment. The method takes advantage of titanium dioxide photocatalysis to decompose organic film in the photo-irradiated region. We show that it can be used to create microdomains of scaffolding proteins, both ex situ and in situ. Neuroscience Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo Takafumi Inoue1, Keishi Narita2, Yuta Nonami1, Hideki Nakamura1, Sen Takeda2 1Department of Life Science and Medical Bioscience, Faculty of Science and Engineering, Waseda University, 2Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine & Engineering, University of Yamanashi In this study, a detailed light microscopic technique was optimized for real-time observation and analysis of the motion of CPEC cilia ex vivo together with an electron microscopic method for ultrastructural analysis.