Gyeongsang National University 5 articles published in JoVE Behavior A Chronic Immobilization Stress Protocol for Inducing Depression-Like Behavior in Mice Hyeonwi Son1, Ju Hwan Yang2, Hyun Joon Kim*1, Dong Kun Lee*3 1Department of Anatomy and Convergence Medical Sciences, Institute of Health Sciences, Bio Anti-aging Medical Research Center, Gyeongsang National University Medical School, 2Department of Biological Sciences, Pusan National University, 3Department of Physiology and Convergence Medical Sciences, Institute of Health Sciences, Gyeongsang National University Medical School This article provides a simplified and standardized protocol for induction of depressive-like behavior in chronically immobilized mice by using a restrainer. In addition, behavior and physiological techniques to verify induction of depression are explained. Bioengineering Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex Rong Li*1, Jinxin Miao*1,2, Zhiqiang Fan1, SeokHwan Song3, Il-keun Kong3,4, Yaohe Wang2,5, Zhongde Wang1 1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London Pronuclear (PN) injection of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein-9 nuclease (CRISPR/Cas9) system is a highly efficient method for producing genetically engineered golden Syrian hamsters. Herein, we describe the detailed PN injection protocol for the production of gene knockout hamsters with the CRISPR/Cas9 system. Chemistry Synthesis of Plant Phenol-derived Polymeric Dyes for Direct or Mordant-based Hair Dyeing Kyung Min Im1, Jong-Rok Jeon1 1Department of Agricultural Chemistry and Food Science & Technology, Institute of Agriculture & Life Science, Gyeongsang National University Here, we present a protocol to use pre-synthesized polymeric products derived from fungal laccase-catalyzed polymerization of plant phenols, either with or without mordant agents (e.g., FeSO4), to induce detergent-resistant keratin hair dyeing within 2.5 hours. Medicine Isolation and Cellular Phenotyping of Mesenchymal Stem Cells Derived from Synovial Fluid and Bone Marrow of Minipigs Won-Jae Lee1,2, Ji-Sung Park1, Si-Jung Jang1, Seung-Chan Lee1, HyeonJeong Lee1, Jae-Hoon Lee1, Gyu-Jin Rho1, Sung-Lim Lee1 1College of Veterinary Medicine, Gyeongsang National University, 2PWG Genetics Pte Ltd. A protocol establishing mesenchymal stem cells (MSCs) isolated from the synovial fluid and bone marrow of minipigs and non-invasively collected using syringe aspiration is presented. Cellular phenotyping was performed using flow cytometry after cell isolation and in vitro cultivation of bone marrow and synovial fluids derived from MSCs. Biology A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response Ritesh Kumar*1, Shu Wei Wu*1, Arya Bagus Boedi Iswanto1, Dhinesh Kumar1, Xiao Han2, Jae-Yean Kim1 1Division of Applied Life Science (BK21 plus program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.