Sogang University 4 articles published in JoVE Engineering Ion-Exchange Membranes for the Fabrication of Reverse Electrodialysis Device Rahul Singh1, Seok Hoon Hong1, Daejoong Kim1 1Department of Mechanical Engineering, Energy Engineering Lab, Sogang University We demonstrate the fabrication of a reverse electrodialysis device using a cation-exchange membrane (CEM) and anion-exchange membrane (AEM) for power generation. Biochemistry Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation Sanggil Kim1, Hyun Soo Lee1 1Department of Chemistry, Sogang University Here, we present a protocol for the genetic incorporation of L-dihydroxyphenylalanine biosynthesized from simple starting materials and its application to protein conjugation. Biochemistry Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition Sanggil Kim1, Wooseok Ko1, Hyunji Park1, Hyun Soo Lee1 1Department of Chemistry, Sogang University Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC). Biology Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide Seonghyun Lee1, Kyubong Jo1 1Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.