King Mongkut's University of Technology Thonburi 1 article published in JoVE Immunology and Infection Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen Muhammad Hatta Prabowo1,2, Thanit Chalermwatanachai3, Werasak Surareungchai2, Patsamon Rijiravanich4,5 1Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia, 2Sensor Technology Laboratory, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, 5 Addressing urgent dengue diagnostic needs, here we introduce a smartphone app-integrated Dengue NS1 Paper-based Analytical Device (DEN-NS1-PAD) for quantifying Dengue NS1 antigen concentration in clinical serum/blood samples. This innovation enhances dengue management by aiding clinical decision-making in various healthcare settings, even resource-limited ones.