National Cheng Kung University 13 articles published in JoVE Biology Induction of Petite Colonies in Candida glabrate via Rose Bengal-Mediated Photodynamic Therapy Cai-Ying Yang*1,2, Jia-Horung Hung*3, Chi-Jung Wu4,5, Zhao-Xiang Wang1,2, Shih-Han Wang6, Hao-Chun Liaw2, I-Huang Lin3, Chun-Keung Yu1,6, Tak-Wah Wong2,7,8 1Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 2Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, 5Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 6Insititute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 7Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 8Center of Applied Nanomedicine, National Cheng Kung University The significance of petite colonies in Candida spp. drug resistance has not been fully explored. Antimicrobial photodynamic therapy (aPDT) offers a promising strategy against drug-resistant fungal infections. This study demonstrates that rose bengal-mediated aPDT effectively deactivates Candida glabrata and induces petite colonies, presenting a unique procedure. Bioengineering Finite Element Analysis Model for Assessing Expansion Patterns from Surgically Assisted Rapid Palatal Expansion Jia-Hong Lin*1, Guan-Lin Wu*2, Chun-Kai Chiu2, Steven Wang3, Chun-Hsi Chung1, Chenshuang Li1 1Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, 2Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, 3Department of Oral and Maxillofacial Surgery/Pharmacology, School of Dental Medicine, University of Pennsylvania A set of novel finite element models of surgically assisted rapid palatal expansion (SARPE) that could perform a clinically required amount of expander activation with various angles of buccal osteotomy was created for further analysis of the expansion patterns of the hemimaxillae in all three dimensions. Biology Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing Szu-Ying Chen1,2, Po-Hsien Huang1,2 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University This protocol provides an easy-to-follow workflow to conduct poly(A) RNA purification, bisulfite conversion, and library preparation using standardized equipment for a biological sample of interest. Neuroscience Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice Chia-Wei Liou*1,2, Tzu-Hsuan Yao*2, Wei-Li Wu1,2 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Physiology, College of Medicine, National Cheng Kung University Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula. Medicine A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage Min-Yu Chang*1,2, Hsi-Hao Wang*1,2, Li-Hung Chen*1, Jhen Gao*1, Shih-Yuan Hung1,2, Yuan-Yow Chiou3,4, Yi-Che Lee1,2,5 1School of Medicine, College of Medicine, I-Shou University, 2Division of Nephrology, Department of Internal Medicine, E-DA Hospital, 3Department of Pediatrics, National Cheng Kung University Hospital, 4Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 5Division of Nephrology, Department of Internal Medicine, E-DA Dachang Hospital The present protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The current model is simple and easy to use compared to other PD animal models. Immunology and Infection Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans Jia-Horung Hung*1,2, Zhao-Xiang Wang*3,4, Yuan-Hsin Lo5, Chaw-Ning Lee3,6, Yun Chang7, Rita Yuchiao Chang7, Chien-Chun Huang3, Tak-Wah Wong3,8,9 1Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, 2Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Dermatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, 5Department of Dermatology, Fu Jen Catholic University Hospital, Fu Jen Catholic University, 6Institute of Clinical Pharmacy and Pharmaceutical Sciences, College of Medicine, National Cheng Kung University, 7School of Medicine, National Cheng Kung University, 8Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 9Center of Applied Nanomedicine, National Cheng Kung University The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro. Immunology and Infection A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease Ting-Jing Shen1,2, Ming-Kai Jhan1,2, Jo-Chi Kao1,2, Min-Ru Ho1,2, Tsung-Ting Tsai1,2, Po-Chun Tseng1,2, Yung-Ting Wang1,2, Chiou-Feng Lin1,2,3 1Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 2Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, 3Center of Infectious Disease and Signaling Research, National Cheng Kung University Here, we present a protocol for creating an immunocompetent ICR (Institute of Cancer Research) murine model of central nervous system infection to display the development of neuropathy. Monitoring acute viral encephalitic disorders by identical disease scores could be performed for showing dengue virus-induced neuropathy in vivo. Biology Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions Yung-Chia Chen1,2, Bu-Miin Huang3 1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 2Department of Anatomy, School of Medicine, Kaohsiung Medical University, 3Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis. Immunology and Infection Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model Yi-Wei Chen1, Wen-Chien Ko1,2, Chang-Shi Chen1,3, Po-Lin Chen2,4 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species. Engineering Uncoupling Coriolis Force and Rotating Buoyancy Effects on Full-Field Heat Transfer Properties of a Rotating Channel Shyy Woei Chang1, Wei-Ling Cai1, Hong-Da Shen1, Kuo-Ching Yu1 1Department of System and Naval Mechatronic Engineering, National Cheng Kung University Here, we present an experimental method for decoupling the interdependent Coriolis-force and rotating-buoyancy effects on full-field heat transfer distributions of a rotating channel. Cancer Research The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues Tsung-Cheng Lin1, Ying-Chih Liao2, Wen-Tsan Chang1,2, Cheng-Han Yang1, Li-Hsin Cheng1, Megan Cheng3, Hung-Chi Cheng1,2 1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3 An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization. Immunology and Infection Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System Cheng-Ju Kuo1,2, Sin-Tian Wang1,2, Chang-Shi Chen1,2 1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization. Behavior An Innovative Running Wheel-based Mechanism for Improved Rat Training Performance Chi-Chun Chen1, Chin-Lung Yang2, Ching-Ping Chang3,4 1Department of Electronic Engineering, National Chin-Yi University of Technology, 2Department of Electrical Engineering, National Cheng Kung University, 3Department of Biotechnology, Southern Taiwan University of Science and Technology, 4Department of Medical Research, Chi Mei Medical Center This study presents an innovative running wheel-based animal mobility system to quantify an effective exercise activity in rats. A rat-friendly testbed is built, using a predefined adaptive acceleration curve, and a high correlation between the effective exercise rate and the infarct volume suggests the protocol's potential for stroke prevention experiments.
