Fudan University Pudong Medical Center 1 article published in JoVE Bioengineering Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution Nan Chen1,2, Kai Zhu1,2, Shiqiang Yan3,4, Junmin Li1,2, Tianyi Pan4, Mieradilijiang Abudupataer1,2, Fazle Alam4, Xiaoning Sun1,2, Li Wang3,4, Chunsheng Wang1,2 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Shanghai Institute of Cardiovascular Diseases, 3Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 4Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College, Fudan University Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells.