Graduate School of Chinese Academy of Medical Sciences and Peking Union Medical College 1 article published in JoVE Biology Dynamic Assessments of Coronary Flow Reserve after Myocardial Ischemia Reperfusion in Mice Ziyu Guo1, Ao Wang2, Yanxiang Gao3, Enmin Xie4, Zixiang Ye1, Yike Li4, Xuecheng Zhao3, Nan Shen1, Jingang Zheng1 1Department of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, 2Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, 3Department of Cardiology, China-Japan Friendship Hospital. No 2 Yinghua Dongjie, 4Graduate School of Chinese Academy of Medical Sciences and Peking Union Medical College The present protocol describes a modified parasternal long-axis view for swift and precise localization of the left anterior descending artery. This approach is designed to be simpler and more user-friendly while facilitating the examination of dynamic changes in coronary flow reserve following myocardial ischemia-reperfusion in mice.