National Institute of Plant Genome Research 2 articles published in JoVE Biology Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology Vadivelmurugan Irulappan1, Muthappa Senthil-Kumar1 1National Institute of Plant Genome Research This study presents methodologies to study the pathomorphological and molecular mechanisms underlying chickpea–Rhizoctonia bataticola interaction. The blotting paper method is useful to rapidly study chickpea genotype responses, while the sick pot-based method can be used to simultaneously impose drought and R. bataticola infection and screen for tolerant genotypes. Biology Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling Deepika Mittal1, Shruti Mishra1, Ramgopal Prajapati1, Jyothilakshmi Vadassery1 1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India A forward genetic screen based on Ca2+ elevation as a read-out leads to identification of genetic components involved in calcium dependent signaling pathways in plants.