InSTEM 1 article published in JoVE Bioengineering A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans Jyoti Dubey1,2,3,4, Sudip Mondal1,5, Sandhya P. Koushika2 1National Centre for Biological Sciences, TIFR, 2Department of Biological Sciences, TIFR, 3InSTEM, 4Manipal Academy of Higher Education, 5Department of Mechanical Engineering, The University of Texas at Austin The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.