CHA University School of Medicine 1 article published in JoVE Medicine A Protocol to Acquire the Degenerative Tenocyte from Humans Soo-Hong Han*1, Hyung Kyung Kim*2, Jong-Ho Ahn*1, Dong Hyeon Lee3, Minjung Baek1, Geunhee Ye1, Joong-Myung Lee1, Kyunghoon Min4, Chihoon Oh1, Soonchul Lee1 1Department of Orthopaedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 2Department of Pathology, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, 3Department of Physiology, CHA University School of Medicine, 4Department of Rehabilitation, CHA Bundang Medical Center, CHA University School of Medicine In vitro use of degenerative tenocytes is essential when investigating the efficacy of novel treatment on tendinopathy. However, most research studies use only the animal model or a healthy tenocyte. We propose the following protocol to isolate human degenerative tenocytes during surgery.