KAIST Institute for Health Science and Technology 1 article published in JoVE Neuroscience In Vivo Whole-Brain Imaging of Zebrafish Larvae Using Three-Dimensional Fluorescence Microscopy Eun-Seo Cho1, Seungjae Han1, Gyuri Kim1, Minho Eom1, Kang-Han Lee2, Cheol-Hee Kim2, Young-Gyu Yoon1,3 1School of Electrical Engineering, KAIST, 2Department of Biology, Chungnam National University, 3KAIST Institute for Health Science and Technology Presented here is a protocol for in vivo whole-brain imaging of larval zebrafish using three-dimensional fluorescence microscopy. The experimental procedure includes sample preparation, image acquisition, and visualization.