Turk-Alman Universitesi 1 article published in JoVE Developmental Biology Development of a Larval Zebrafish Infection Model for Clostridioides difficile Junkai Li1, Can M. Ünal2, Kazuhiko Namikawa1, Michael Steinert3,4,5, Reinhard W. Köster1 1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.