Vrije Universiteit Brussel View Institution's Website 5 articles published in JoVE Medicine Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol Erich Hohenauer1,2,3, Peter Clarys3, Jean-Pierre Baeyens2,3,4, Ron Clijsen1,2,3 1Department of Business Economics, Health and Social Care, University of Applied Sciences and Arts of Southern Switzerland, 2University College Physiotherapy "Thim van der Laan", 3Department of Movement and Sport Sciences, Vrije Universiteit Brussel, 4Faculty of Medicine and Health Sciences, University of Antwerp This protocol describes the procedure for non-invasive recovery assessments during a 72 h recovery period. This protocol induces muscular exhaustion of the frontal thighs using countermovement jumps and uses either local cold-cuff or thermoneutral-cuff application as a recovery modality. Engineering Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron Bernd Helber1,2, Olivier Chazot1, Annick Hubin2, Thierry E. Magin1 1Aeronautics and Aerospace Department, von Karman Institute for Fluid Dynamics, 2Research Group Electrochemical and Surface Engineering, Vrije Universiteit Brussel Development of new ablative materials and their numerical modeling requires extensive experimental investigation. This protocol describes procedures for material response characterization in plasma flows with the core techniques being non-intrusive methods to track the material recession along with the chemistry in the reactive boundary layer by emission spectroscopy. Medicine Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation Sofie De Groef1, Gunter Leuckx1, Naomi Van Gassen1, Willem Staels1, Ying Cai1, Yixing Yuchi1, Violette Coppens1, Nico De Leu1, Yves Heremans1, Luc Baeyens1, Mark Van de Casteele1, Harry Heimberg1 1Diabetes Research Center, Vrije Universiteit Brussel This protocol describes an injury model involving the surgical ligation of the pancreatic duct in the adult mouse pancreas, resulting in severe injury that establishes an environment that allows beta cell neogenesis and proliferation. This model can be used as a tool to study mechanisms involved in beta cell formation. Medicine Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy Charles A. Kunos1, Jeffrey M. Fabien1, John P. Shanahan1, Christine Collen2, Thierry Gevaert2, Kenneth Poels2, Robbe Van den Begin2, Benedikt Engels2, Mark De Ridder2 1Department of Radiation Oncology, Summa Cancer Institute, 2Department of Radiation Oncology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel Stereotactic ablative body radiation therapy (SBRT) involves the precise delivery of high-dose radiation to cancer tumor targets. A novel SBRT platform offers a first-of-its-kind gimbaled radiation accelerator mounted within a pivoting O-ring gantry capable of dynamic image-guided tumor tracking. Here, we describe dynamic tumor tracking for lung targets. Biology A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example Lise Schotte1, Bart Rombaut1, Bert Thys1 1Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel In this article, a simple, quantitative, liquid phase affinity capture assay is presented. It is a reliable technique based on the interaction between magnetic beads and tagged proteins (e.g. nanobodies) on one hand and the affinity between the tagged protein and a second, labeled protein (e.g. poliovirus) on the other.