University of Erlangen-Nuremberg View Institution's Website 8 articles published in JoVE Medicine How to Administer Near-Infrared Spectroscopy in Critically ill Neonates, Infants, and Children Nora Bruns1, Julia Moosmann2, Frank Münch3, Christian Dohna-Schwake1, Joachim Woelfle4, Robert Cesnjevar3, Sven Dittrich2, Ursula Felderhoff-Müser1, Hanna Müller4 1Department of Pediatrics I - Neonatology, Pediatric Intensive Care, Pediatric Neurology, University Hospital Essen, University of Duisburg-Essen, 2Department of Pediatric Cardiology, University Hospital Erlangen, University of Erlangen-Nürnberg, 3Department of Pediatric Cardiac Surgery, University Hospital Erlangen, University of Erlangen-Nürnberg, 4Division of Neonatology and Pediatric Intensive Care, Department of Pediatrics, University Hospital Erlangen, University of Erlangen-Nürnberg This protocol is designed to assist clinicians to measure regional tissue oxygenation at different body sites in infants and children. It can be used in situations where tissue oxygenation is potentially compromised, particularly during cardiopulmonary bypass, when using non-pulsatile cardiac-assist devices, and in critically ill neonates, infants and children. Immunology and Infection Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease Emily Becker1, Sebastian Schramm1, Marie-Theres Binder1, Clarissa Allner1, Maximilian Wiendl1, Clemens Neufert1, Imke Atreya1, Markus Neurath1, Sebastian Zundler1 1Department of Medicine 1, University of Erlangen-Nuremberg Dynamic adhesion of immune cells to the vessel wall is a prerequisite for gut homing. Here, we present a protocol for a functional in vitro assay for the impact analysis of anti-integrin antibodies, chemokines or other factors on the dynamic cell adhesion of human cells using addressin-coated capillaries. Engineering Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions José R. Agudo1, Jiwon Han1, Jinyoung Park1, Sinyoung Kwon1, Soebiakto Loekman2, Giovanni Luzi1, Christoph Linderberger3, Antonio Delgado1,4, Andreas Wierschem4 1Institute of Fluid Mechanics, FAU Busan Campus, University of Erlangen-Nuremberg, 2Institute of Chemical Reaction Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 3Institute of Bioprocess Engineering, FAU Busan Campus, University of Erlangen-Nuremberg, 4Institute of Fluid Mechanics, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are presented. Neuroscience Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies Andreas Feigenspan1, Norbert Zsolt Babai1 1Department of Biology, Friedrich-Alexander-University (FAU) Erlangen-Nürnberg We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques. Engineering Adsorption Device Based on a Langatate Crystal Microbalance for High Temperature High Pressure Gas Adsorption in Zeolite H-ZSM-5 Wenjin Ding1, Giulia Baracchini1, Michael Klumpp2, Wilhelm Schwieger2, Roland Dittmeyer1 1Institute for Micro Process Engineering, Karlsruhe Institute of Technology (KIT), 2Institute of Chemical Reaction Engineering, University Erlangen-Nürnberg (FAU) A protocol for high-temperature and high-pressure gas adsorption measurements on zeolite H-ZSM-5 using an adsorption measurement device based on a langatate crystal microbalance is presented. Prior to the adsorption measurements, the synthesis of zeolite H-ZSM-5 on the langatate crystal microbalance sensor by the steam-assisted crystallization (SAC) method is demonstrated. Bioengineering Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions Kristin Derlig1, Andreas Gießl2, Johann Helmut Brandstätter2, Ralf Enz1, Regina Dahlhaus1 1Institute for Biochemistry, Emil-Fischer Centre, University of Erlangen-Nuremberg, 2Department of Biology, Animal Physiology, University of Erlangen-Nuremberg Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function. Medicine Surgical Fixation of Sternal Fractures: Preoperative Planning and a Safe Surgical Technique Using Locked Titanium Plates and Depth Limited Drilling Stefan Schulz-Drost1, Pascal Oppel1, Sina Grupp1, Sonja Schmitt1, Roman Th. Carbon2, Andreas Mauerer3, Friedrich F. Hennig1, Thomas Buder4 1Orthopedic and Trauma Surgery, University Hospital Erlangen, 2Pediatric Surgery, University Hospital Erlangen, 3Orthopedic and Trauma Surgery, St.-Theresien Hospital, 4Institute of Anatomy I, University Erlangen-Nuremberg Here we present a method to stabilize sternal fractures by using locked titanium plates in a low profile design. Performing subperiosteal dissection along the sternum while reducing the fracture, using depth limited drilling, and fixing the plates provides a safe surgical way. Neuroscience A Low Cost Setup for Behavioral Audiometry in Rodents Konstantin Tziridis1, Sönke Ahlf1, Holger Schulze1 1Experimental Otolaryngology, University of Erlangen-Nuremberg A fast and inexpensive method for the behavioral determination of hearing parameters like hearing thresholds, hearing impairments or phantom perceptions (subjective tinnitus) is described. It uses pre-pulse inhibition of the acoustic startle response and can be easily implemented in a personal computer using a programmable AD/DA-converter and a piezo sensor.