2bind GmbH 1 article published in JoVE Biochemistry Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis Clemens Entzian1, Thomas Schubert1 12bind GmbH MicroScale Thermophoresis (MST) is a sensitive technology to characterize aptamer-target interactions. This manuscript describes an MST protocol to characterize aptamer-small molecule interactions.