Agilent Technologies 1 article published in JoVE Biology Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C Antonia Hauth1, Rafael Galupa1, Nicolas Servant2, Laura Villacorta1, Kai Hauschulz3, Joke Gerarda van Bemmel4, Agnese Loda1, Edith Heard1,5 1EMBL: European Molecular Biology Laboratory, 2Institut Curie, 3Agilent Technologies, 4Genmab BV, 5Collège de France This protocol describes the Capture Hi-C method used to characterize the 3D organization of megabased-sized targeted genomic regions at high-resolution, including boundaries of topologically associating domains (TADs) and long-range chromatin interactions between regulatory and other DNA sequence elements.