University of Tartu 2 articles published in JoVE Neuroscience Establishing Mixed Neuronal and Glial Cell Cultures from Embryonic Mouse Brains to Study Infection and Innate Immunity Alistair Gamble1, Maria Suessmilch1, Anniek Bonestroo1, Andres Merits2, Gerard J. Graham1, Jonathan Cavanagh1, Julia M. Edgar1, Marieke Pingen1 1School of Infection and Immunity, University of Glasgow, 2Faculty of Science and Technology, Institute of Technology, University of Tartu This protocol presents a unique way of generating central nervous system cell cultures from embryonic day 17 mouse brains for neuro(immuno)logy research. This model can be analyzed using various experimental techniques, including RT-qPCR, microscopy, ELISA, and flow cytometry. Engineering Fabrication of Carbon-Based Ionic Electromechanically Active Soft Actuators Pille Rinne1, Inga Põldsalu1, Herman Klas Ratas1, Karl Kruusamäe1, Urmas Johanson1, Tarmo Tamm1, Kaija Põhako-Esko1, Andres Punning1, Anna-Liisa Peikolainen1, Friedrich Kaasik1, Indrek Must1, Daan van den Ende2, Alvo Aabloo1 1Intelligent Materials and Systems Lab, Institute of Technology, University of Tartu, 2Smart Interfaces & Modules Department, Philips Research This article describes a fast and simple manufacturing process of ionic electromechanically active composite materials for actuators in biomedical, biomimetic and soft robotics applications. The key fabrication steps, their importance for the actuators' final properties, and some of the main characterization techniques are described in detail.