Agrobio S.L. 1 article published in JoVE Engineering Glass-Based Devices to Generate Drops and Emulsions Josefa Guerrero1, Javier Rojo2, Alexis de la Cotte2, Luis Manuel Aguilera-Sáez3, Enric Vila3, Alberto Fernandez-Nieves2,4,5 1Department of Chemistry and Physics, Augusta University, 2Department of Condensed Matter Physics, University of Barcelona, 3Agrobío S.L., 4ICREA - Institució Catalana de Recerca i Estudis Avançats, 5Institute of Complex Systems (UBICS), University of Barcelona Here, a protocol to manufacture glass-based microfluidic devices used for generating highly monodisperse emulsions with controlled drop size is presented.