University Hospital of Nice 1 article published in JoVE Medicine Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation Carmen Mariana Aanei1, Marie-Christine Jacob2, Richard Veyrat-Masson3, Tiphanie Picot1, Maria Alessandra Rosenthal-Allieri4, Anne-Catherine Lhoumeau5, Michel Ticchioni4, Florent Dumezy6, Lydia Campos Catafal1 1Department of Hematology, University Hospital of Saint-Etienne, 2Department of Immunology, University Hospital of Grenoble-Alpes, 3Department Hematology, University Hospital of Clermont-Ferrand, 4Department of Immunology, University Hospital of Nice, 5Department of Biopathology, Institute Paoli-Calmettes, 6Department of Hematology, University Hospital of Lille The MDS diagnosis is difficult in the absence of morphological criteria or non-informative cytogenetics. MFC could help refine the MDS diagnostic process. To become useful for clinical practice, the MFC analysis must be based on parameters with sufficient specificity and sensitivity, and data should be reproducible between different operators.