Ksilink 1 article published in JoVE Neuroscience Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons Amélie Weiss1, Peter Sommer1, Johannes H. Wilbertz1 1Ksilink This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical modulations.