John Innes Centre, Norwich Research Park 1 article published in JoVE Immunology and Infection Fluorescence Time-lapse Imaging of the Complete S. venezuelae Life Cycle Using a Microfluidic Device Susan Schlimpert1, Klas Flärdh2, Mark J. Buttner1 1Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, 2Department of Biology, Lund University Streptomyces are characterized by a complex life cycle that has been experimentally challenging to study by cell biological means. Here we present a protocol to perform fluorescence time-lapse microscopy of the complete life cycle by growing Streptomyces venezuelae in a microfluidic device.