Loughborough University 4 articles published in JoVE Biology Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry Rebecca Lees*1, Robert Tempest*1, Alice Law*1, Dimitri Aubert*1, Owen G. Davies*2, Soraya Williams*2, Nick Peake*3, Ben Peacock*1 1NanoFCM Co., Ltd, 2School of Sport, Exercise and Health Sciences, Loughborough University, 3Biomolecular Sciences Research Centre, Sheffield Hallam University The latest generation of EV characterization tools are capable of single EV analysis across multiple parameters simultaneously. Nano-flow cytometry measures all biological particles larger than 45 nm without labeling and identifies specific characteristics of subpopulations by a variety of fluorescent labeling techniques. Bioengineering Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example Emma L. C. J. Blundell1, Robert Vogel2,3, Mark Platt1 1Department of Chemistry, School of Science, Loughborough University, 2Izon Science Limited, 3School of Mathematics and Physics, The University of Queensland Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles. Chemistry A Simple, Low-cost, and Robust System to Measure the Volume of Hydrogen Evolved by Chemical Reactions with Aqueous Solutions Paul Brack1, Sandie Dann1, K. G. Upul Wijayantha1, Paul Adcock2, Simon Foster2 1Department of Chemistry, Loughborough University, 2Intelligent Energy Ltd The study of methods to generate on-demand hydrogen for fuel cells continues to grow in importance. However, systems to measure hydrogen evolution from the reaction of chemicals with water can be complicated and expensive. This article details a simple, low-cost, and robust method to measure the evolution of hydrogen gas. Bioengineering Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall Jack C. Bridge1, Jonathan W. Aylott2, Christopher E. Brightling5, Amir M. Ghaemmaghami3, Alan J. Knox4, Mark P. Lewis6, Felicity R.A.J. Rose1, Gavin E. Morris1 1Division of Drug Delivery and Tissue Engineering, University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences, University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences, University of Nottingham, 5NIHR Respiratory Biomedical Research Unit, University of Leicester, 6School of Sport, Exercise, and Health Sciences, Loughborough University Advancements in biomaterial technologies enable the development of three-dimensional multi-cell-type constructs. We have developed electrospinning protocols to produce three individual scaffolds to culture the main structural cells of the airway to provide a 3D in vitro model of the airway bronchiole wall.