Protatonce Ltd, Demokritos Science Park 1 article published in JoVE Bioengineering A GMP-Compliant Procedure for the Generation of Gene-Modified T cells Nikolaos Savvopoulos1, Klearchos Stampolitis1, Georgios Alexandropoulos1, Dionysia Kefala1, Memnon Lysandrou1, Vassiliki Zacharioudaki1, Nikos Tsolakos2, Alexandros Spyridonidis1 1Institute of Cell Therapy, Bone Marrow Transplantation Lab, Department of Medicine, University of Patras, 2Protatonce Ltd, Demokritos Science Park This protocol outlines the process of transducing primary human T cells with a gene of interest, ensuring compatibility with Good Manufacturing Practice (GMP) standards.