Semmelweis University View Institution's Website 12 articles published in JoVE Neuroscience In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse Richárd Sinkó1,2, Petra Mohácsik1, Csaba Fekete*3, Balázs Gereben*1 1Laboratory of Molecular Cell Metabolism, Institute of Experimental Medicine, 2János Szentágothai PhD School of Neurosciences, Semmelweis University, 3Laboratory of Integrative Neuroendocrinology, Institute of Experimental Medicine The Thyroid Hormone Action Indicator mouse model was developed to enable tissue-specific quantification of local thyroid hormone action using its endogenous regulatory machinery. Recently, it has been shown that the model is suitable for characterizing endocrine-disrupting chemicals interacting with thyroid hormone economy, both by ex vivo and in vivo methodologies. Medicine Dynamic Navigation for Dental Implant Placement Gabor Tamas Pinter1, Roland Decker1, Gabor Szenasi2, Peter Barabas1, Tamas Huszar1 1Department of Oro-Maxillofacial Surgery and Stomatology, Faculty of Dentistry, Semmelweis University, 2Doctoral School Of Theoretical And Translational Medicine, Schools of PhD Studies, Semmelweis University Dynamic computer-aided implant surgery (DCAIS) is a controlled implant surgical placement method performed without a surgical template using optical control. The real-time intraoperative control of movement and position of the surgical device simplifies the procedure and gives more freedom to the surgeon, providing similar precision as static navigation methods. Neuroscience A Minimally Invasive, Fast Spinal Cord Lateral Hemisection Technique for Modeling Open Spinal Cord Injuries in Rats Krisztián B. Csomó1, Gábor Varga2,3, Andrea A. Belik4, László Hricisák5, Zoltán Borbély2, Gábor Gerber6 1Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University, 2Department of Oral Biology, Faculty of Dentistry, Semmelweis University, 3Centre for Translational Medicine, Semmelweis University, 4Department of Molecular Biology, Faculty of Medicine, Semmelweis University, 5Institute of Translational Medicine, Faculty of Medicine, Semmelweis University, 6Department of Anatomy, Histology and Embryology, Faculty of Medicine, Semmelweis University Here, we describe a new, fast technique modeling open spinal cord injury in rats that eliminates laminectomy. Lateral hemisection is performed while viewing through a microscope. The technique is versatile and can also be used in the cervical, thoracic, and lumbar regions of the spinal cord of other animals. Medicine Echocardiographic Evaluation of Atrial Communications before Transcatheter Closure Andrea Ágnes Molnár*1, Pál Ábrahám*1, Béla Merkely1, Sándor Nardai1 1Heart and Vascular Center, Semmelweis University Transthoracic (TTE) and transesophageal (TEE) echocardiography represent the basic imaging tools for interatrial septum examination. Three dimensional (3D) TEE provides incremental information in the assessment of the interatrial septum. Further advanced echocardiography techniques using speckle tracking echocardiography are applied for sensitive volumetric and functional assessment of the heart chambers. Medicine Time-Resolved, Dynamic Computed Tomography Angiography for Characterization of Aortic Endoleaks and Treatment Guidance via 2D-3D Fusion-Imaging Marton Berczeli1,2, Ponraj Chinnadurai1,3, Su Min Chang4, Alan B. Lumsden1 1Department of Cardiovascular Surgery, Houston Methodist Hospital, 2Department of Vascular and Endovascular Surgery, Semmelweis University, 3Advanced Therapies, Siemens Medical Solutions USA Inc., 4Department of Cardiology, Houston Methodist Hospital Dynamic computed tomography angiography (CTA) imaging provides additional diagnostic value in characterizing aortic endoleaks. This protocol describes a qualitative and quantitative approach using time-attenuation curve analysis to characterize endoleaks. The technique of integrating dynamic CTA imaging with fluoroscopy using 2D-3D image fusion is illustrated for better image guidance during treatment. Medicine Digital Hybrid Model Preparation for Virtual Planning of Reconstructive Dentoalveolar Surgical Procedures Daniel Palkovics1, Eleonora Solyom1, Balint Molnar1, Csaba Pinter*2, Peter Windisch*1 1Department of Periodontology, Semmelweis University, 2Empresa de Base Tecnológica Internacional de Canarias, S.L. (EBATINCA) A workflow for creating three-dimensional (3D) virtual hybrid models has been designed based on cone-beam computed tomography dataset and intraoral optical scans utilizing radiographic image segmentation methods and free-form surface modeling. Digital models are used for the virtual planning of reconstructive dentoalveolar surgical procedures. Medicine Post-Myocardial Infarction Heart Failure in Closed-chest Coronary Occlusion/Reperfusion Model in Göttingen Minipigs and Landrace Pigs Gábor B Brenner*1,2, Zoltán Giricz*1,2, Rita Garamvölgyi3,4, András Makkos1, Zsófia Onódi1, Nabil V Sayour1, Tamás G Gergely1, Tamás Baranyai1, Örs Petneházy3,4, Dénes Kőrösi3, Gergő P. Szabó3, Hajnalka Vago5, Zsófia Dohy5, Csilla Czimbalmos5, Béla Merkely5, Swetlana Boldin-Adamsky6, Elena Feinstein6, Iván G Horváth7, Péter Ferdinandy1,2 1Department of Pharmacology and Pharmacotherapy, Semmelweis University, 2Pharmahungary Group, 3Diagnostic, Radiation Oncology, Research and Teaching Center, Moritz Kaposi Somogy County Teaching Hospital, 4Faculty of Agricultural and Environmental Sciences, Kaposvar University, 5Heart and Vascular Center, Semmelweis University, 6Quark Pharmaceuticals, Inc, 7Heart Institute, Medical School, University of Pécs The overall goal of the current study is to present the techniques of induction of myocardial infarction (MI) and post-myocardial infarction heart failure (post-MI HF) in closed-chest, adult Göttingen minipigs and the characterization of post-MI HF model in Göttingen minipigs as compared to Landrace pigs. Medicine Morphological and Functional Assessment of the Right Ventricle Using 3D Echocardiography Bálint Károly Lakatos1, Márton Tokodi1, Erika Kispál1, Béla Merkely1, Attila Kovács1 1Semmelweis University Heart and Vascular Center Here, we provide a step-by-step acquisition and analysis protocol for the 3D volumetric assessment of the right ventricle, mainly focusing on the practical aspects that maximize the feasibility of this technique. Bioengineering Evaluation of Left Ventricular Structure and Function using 3D Echocardiography Adrienn Ujvári1, Bálint Károly Lakatos1, Márton Tokodi1, Alexandra Fábián1, Béla Merkely1, Attila Kovács1 1Heart and Vascular Center, Semmelweis University In this article, we provide a step-by-step acquisition and analysis protocol for the volumetric assessment and speckle-tracking analysis of the left ventricle by 3D echocardiography, particularly focusing on practical aspects that maximize the feasibility of this technique. Medicine A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva Reka Fazekas1, Eszter Molnár1, Barbara Mikecs1, Zsolt Lohinai1, János Vág1 1Department of Conservative Dentistry, Faculty of Dentistry, Semmelweis University This study introduces a protocol for measuring microcirculation in human oral mucosa by laser speckle contrast imaging. The monitoring of wound healing after vestibuloplasty combined with a xenogenic collagen graft is presented on a clinical case. Cancer Research In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues József Murányi1,2, Attila Varga1, Bianka Gurbi1, Pál Gyulavári1, Gábor Mező3, Tibor Vántus2 1MTA-SE Pathobiochemistry Research Group, Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry, Eötvös Loránd University Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines. Medicine Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model Attila Cselenyák1, Zsolt Benko1, Mónika Szepes1, Levente Kiss1, Zsombor Lacza1 1Institute of Human Physiology and Clinical Experimental Research, Semmelweis University We demonstrate how to set up an in vitro ischemia/reperfusion model and how to evaluate the effect of stem cell therapy on postischemic cardiac cells.