Erasmus University Medical Center - Sophia Children's Hospital 1 article published in JoVE Developmental Biology Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing Naomi J. M. Kakiailatu1, Laura E. Kuil1,2, Eric Bindels3, Joke T. M. Zink3, Michael Vermeulen3, Veerle Melotte4, Maria M. Alves1 1 Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis.