AstraZeneca 1 article published in JoVE Biology Isolation and Culture of Human Mature Adipocytes Using Membrane Mature Adipocyte Aggregate Cultures (MAAC) Ida Alexandersson1, Matthew J. Harms1, Jeremie Boucher1,2,3 1Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, 2The Lundberg Laboratory for Diabetes Research, University of Gothenburg, 3Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg Membrane mature adipocyte aggregate culture (MAAC) is a new method to culture mature human adipocytes. Here we detail how to isolate adipocytes from human adipose and how to set up MAAC.