Swedish University of Agricultural Sciences View Institution's Website 7 articles published in JoVE Behavior A Wind Tunnel for Odor Mediated Insect Behavioural Assays Geir K. Knudsen1, Marco Tasin2, Anders Aak3, Gunda Thöming1 1Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (Nibio), 2Integrated Plant Protection Unit, Department of Plant Protection Biology, Swedish University of Agricultural Science, 3Department of Pest Control, Norwegian Institute of Public Health Here, we describe the construction and use of a wind tunnel for odor mediated behavioural assays with insects. The wind tunnel design facilitates the release of odor sources by several methods, with and without visual stimuli. Wind tunnel experiments are important methods to identify behaviorally active volatile chemicals. Bioengineering Film Extrusion of Crambe abyssinica/Wheat Gluten Blends Mikael Gällstedt1, Henrik Pettersson2, Therese Johansson2, William R. Newson3, Eva Johansson3, Mikael S. Hedenqvist4 1SIG Combibloc, 2Innventia, 3Department of Plant Breeding, The Swedish University of Agricultural Sciences, 4Fibre and Polymer Technology, KTH Royal Institute of Technology The side stream from plant oil production of Crambe abyssinica has limited value. The purpose of this study was to find methods for extruding materials based on this side stream, demonstrating that products with a higher value can be produced. The extrudates were found to have promising properties. Environment Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water Lutz Ahrens1, Atlasi Daneshvar1,2, Anna E. Lau1,2, Jenny Kreuger1,2 1Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, 2Center for Chemical Pesticides, Swedish University of Agricultural Sciences A protocol about the characterization and application of five different passive sampling devices is presented. Biology Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam Stefano Rubino1,4, Petter Melin3, Paul Spellward2, Klaus Leifer1 1Department of Engineering Sciences, Uppsala University, 2Gatan Inc., 3Department of Microbiology, Swedish University of Agricultural Sciences, 4Physics Department, University of Oslo Cryo Electron Microscopes, either Scanning (SEM) or Transmission (TEM), are widely used for characterization of biological samples or other materials with a high water content1. A SEM/Focused Ion Beam (FIB) is used to identify features of interest in samples and extract a thin, electron-transparent lamella for transfer to a cryo-TEM. Biology Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival Per Wessman1, Sebastian Håkansson1, Klaus Leifer2, Stefano Rubino2 1Department of Microbiology, Uppsala Biocenter, Swedish University of Agricultural Sciences, 2Department of Engineering Sciences, Uppsala University Freeze-drying is often an easy and convenient way to obtain dry products of viable bacterial cells. An issue of the process is cell survival. We detail here a procedure to investigate how cell survival during freeze-drying is influenced by the properties of the formulation used. Biology Quantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast Pernilla Bjerling1, Ida Olsson1,2, Xi'nan Meng1 1Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, University of Uppsala, 2Department of Microbiology, Swedish University of Agricultural Sciences The fission yeast, Schizosaccharomyces pombe, is a good model system to study basic cellular processes. Here we describe a method to perform quantitative live cell analysis of fission yeast. In this particular experiment we focus on organisation of the genome within the cell nucleus, but the method can also be used to study cytosolic factors. Biology Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species Anna Karlgren1, Jenny Carlsson2, Niclas Gyllenstrand2, Ulf Lagercrantz1, Jens F. Sundström2 1Department of Evolutionary Functional Genomics, Evolutionary Biology Center, Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Sciences We describe a modified DIG in situ hybridization protocol, which is fast and applicable on a wide range of plant species including Norway spruce. With just a few adjustments, including altered RNase treatment and proteinase K concentration, the protocol may be used in studies of different tissues and species.