Westfälische Wilhelms-University Münster View Institution's Website 4 articles published in JoVE Medicine Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET Alexander Grabner*1, Dominik Kentrup*1, Uta Schnöckel2, Gert Gabriëls1, Rita Schröter1, Hermann Pavenstädt1, Otmar Schober2, Eberhard Schlatter1, Michael Schäfers*3, Stefan Reuter*1 1Department of Internal Medicine D, Experimental Nephrology, University of Münster, 2Department of Nuclear Medicine, University of Münster, 3European Institute for Molecular Imaging, University of Münster We herein present a rat renal transplantation model to non-invasively assess acute allograft rejection using positron emission tomography with 18F-fluorodeoxyglucose. Biology Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format Miao Zhang1, Hans R. Schöler1,2, Boris Greber1 1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty, University of Münster Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner. Engineering Controlling the Size, Shape and Stability of Supramolecular Polymers in Water Pol Besenius1, Isja de Feijter2, Nico A.J.M. Sommerdijk3, Paul H.H. Bomans3, Anja R. A. Palmans2 1Organic Chemistry Institute and CeNTech, Westfälische Wilhelms-Universität Münster, 2Laboratory of Macromolecular and Organic Chemistry, Institute for Complex Molecular Systems, Eindhoven University of Technology, 3Laboratory of Materials and Interface Chemistry and Soft Matter Research Unit, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology The goal of this experiment is to determine and control the size, shape and stability of self-assembled discotic amphiphiles in water. For aqueous based supramolecular polymers such level of control is very difficult. We apply a strategy using both repulsive and attractive interactions. The experimental techniques applied to characterize this system are broadly applicable. Medicine Implantation of a Carotid Cuff for Triggering Shear-stress Induced Atherosclerosis in Mice Michael T. Kuhlmann1, Simon Cuhlmann2,3, Irmgard Hoppe1, Rob Krams3, Paul C. Evans2, Gustav J. Strijkers4, Klaas Nicolay4, Sven Hermann1, Michael Schäfers1 1European Institute for Molecular Imaging, Westfälische Wilhelms-University Münster, 2British Heart Foundation Cardiovascular Sciences Unit, Imperial College London, 3Department of Bioengineering, Imperial College London, 4Biomedical Engineering, Eindhoven University of Technology The constricting cuff presented in this article is designed to induce atherosclerosis in the murine common carotid artery. Due to the conical shape of its inner lumen the implanted cuff generates well-defined regions of low, high and oscillatory shear stress triggering the development of atherosclerotic lesions of different inflammatory phenotypes.