Ecole Superieure d'Ingenieurs Leonard de Vinci ESILV 1 article published in JoVE Bioengineering Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering Deborah Pagliaccia*1,6, Douglas Hill*2, Emily Dang1, Gerardo Uribe1, Agustina De Francesco1, Ryan Milton2, Anthony De La Torre2, Axel Mounkam2, Tyler Dang1, Sohrab Bodaghi1, Irene Lavagi-Craddock1, Alexandra Syed1, William Grover3, Adriann Okamba4,5, Georgios Vidalakis*1 1Department of Microbiology and Plant Pathology, University of California Riverside, 2Technology Evolving Solutions (TES), 3Department of Bioengineering, University of California Riverside, 4 We engineered, fabricated, and validated an instrument that rapidly processes phloem-rich bark citrus budwood tissues. Compared to current methods, the budwood tissue extractor (BTE) has increased sample throughput and decreased the required labor and equipment costs.