CytoGnomix 1 article published in JoVE Genetics Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation Ben Shirley*1, Yanxin Li*1, Joan H.M. Knoll1,2, Peter K. Rogan1,3 1CytoGnomix Inc., 2Department of Pathology and Laboratory Medicine, Western University, 3Department of Biochemistry, Western University The cytogenetic dicentric chromosome (DC) assay quantifies exposure to ionizing radiation. The Automated Dicentric Chromosome Identifier and Dose Estimator software accurately and rapidly estimates biological dose from DCs in metaphase cells. It distinguishes monocentric chromosomes and other objects from DCs, and estimates biological radiation dose from the frequency of DCs.